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VENA8 ENDOTHELIAL+微流體芯片的主要特點(diǎn)：

用于細(xì)胞滾動(dòng)、粘附分子研究或細(xì)胞間相互作用研究

適用于各種細(xì)胞懸浮液(原代和細(xì)胞株)包括T細(xì)胞、單核細(xì)胞、外周血單個(gè)核細(xì)胞、中性粒細(xì)胞、嗜酸性粒細(xì)胞、血小板和全血(肝素化)。

Vena8熒光+ TM和 Vena8TM：可以用標(biāo)準(zhǔn)移液器將一系列的粘附因子包覆毛細(xì)管外壁。不同的黏附分子包括VCAM(血管細(xì)胞粘附因子)，ICAM(細(xì)胞間粘附因子)，MAdCAM，纖維連接蛋白，vWF(人血管性血友病因子/瑞斯托霉素輔因子)、膠原蛋白、纖維蛋白原等。

Vena8內(nèi)皮細(xì)胞+ TM和VenaECTM：容易種植和培養(yǎng)各種內(nèi)皮細(xì)胞(層)包括HUVEC(人臍靜脈血管內(nèi)皮細(xì)胞)，HMVEC(人微血管內(nèi)皮細(xì)胞)，HCAECs(人冠狀動(dòng)脈內(nèi)皮細(xì)胞)等等。

生物芯片具有良好的光學(xué)特性，可在顯微鏡下進(jìn)行清晰的觀察和進(jìn)一步的研究。

剪切應(yīng)力范圍：0.05-20 dyne/ cm2，可以通過(guò)MirusTM Nanopump精確控制。

剪切應(yīng)力的大小及連續(xù)變化的參數(shù)可以設(shè)置。

可在流動(dòng)狀態(tài)下實(shí)時(shí)成像。

The Vena8 Endothelial+™ biochip is ideal for cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow. Ideal for use with PCR. Compatible with brightfield， phase contrast imaging and fluorescence microscopy.

Features:

• Cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow.

• Cells cultured within the biochip channels may be retrieved post-assay for subsequent studies (up to 320，000 cells); e.g. PCR. Substrate is removed from biochip and cells are scraped off.

• Suitable for whole blood， primary cells， rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.

• No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.

• No luer lock connections which increase dead volume. Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable. Dead volume is at input port ~ 0.1μL.

Applications: Flow assays using minimal sample volumes (whole blood， cell suspensions， proteins etc.); brightfield， phase contrast and fluorescence microscopy.

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